IN-VIVO PATHOGENICITY OF AEROLYSIN TOXIN OF AEROMONAS HYDROPHILA ISOLATED FROM DIARRHEA PATIENTS IN THI-QAR PROVINCE.

Aeromonas species are highly distributed in the world and causative agent for fish, animal and human diseases. The study concluded 255 fecal sampler (168 sample were collected from patient suffering from diarrhea and 82 sample from apparently healthy). The Aeromonas hydrophila isolates were identified depended on culture media and biochemical methods for I field A.hydrophila; the PCR technique used to detection thewhole forming Toxin (aerolysin) gene. The results revealed that 23 isolates of A. hydrophila were identified from diarrheal patients, while 18 isolates from apparently healthy. Aerolysin gene was detected in 9 isolates only. To determine the in vivo pathogenicity of A. hydrophila, forty two mice were used to detect LD₅₀ value, then determined the histological changes of organs including intestine and liver were obtained for re-isolation and histopathological examination. Lethal dose of also demonstrated in mice was 0.9×1000000000. The microscopic examination of histopathological sections of intestine of infected mice after LD₅₀ experiment showed that preserver of hyperplasia of lymphoid tissue with thickening in villi, congestion of central vein of liver, mild inflammation in periportal area, fatty changes and parenchymal cell of liver. [ABSTRACT FROM AUTHOR]