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Gill transcriptomes reveal expression changes of genes related with immune and ion transport under salinity stress in silvery pomfret (Pampus argenteus).
- Source :
- Fish Physiology & Biochemistry; Aug2020, Vol. 46 Issue 4, p1255-1277, 23p
- Publication Year :
- 2020
-
Abstract
- Salinity is a major ecological factor in the marine environment, and extremely important for the survival, development, and growth of fish. In this study, gill transcriptomes were examined by high-throughput sequencing at three different salinities (12 ppt as low salinity, 22 ppt as control salinity, and 32 ppt as high salinity) in an importantly economical fish silvery pomfret. A total of 187 genes were differentially expressed, including 111 up-regulated and 76 down-regulated transcripts in low-salinity treatment group and 107 genes differentially expressed, including 74 up-regulated and 33 down-regulated transcripts in high-salinity treatment group compared with the control group, respectively. Some pathways including NOD-like receptor signaling pathway, cytokine-cytokine receptor interaction, Toll-like receptor pathway, cardiac muscle contraction, and vascular smooth muscle contraction were significantly enriched. qPCR analysis further confirmed that mRNA expression levels of immune (HSP90A, IL-1β, TNFα, TLR2, IP-10, MIG, CCL19, and IL-11) and ion transport–related genes (WNK2, NPY2R, CFTR, and SLC4A2) significantly changed under salinity stress. Low salinity stress caused more intensive expression changes of immune-related genes than high salinity. These results imply that salinity stress may affect immune function in addition to regulating osmotic pressure in silvery pomfret. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 09201742
- Volume :
- 46
- Issue :
- 4
- Database :
- Complementary Index
- Journal :
- Fish Physiology & Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 144476207
- Full Text :
- https://doi.org/10.1007/s10695-020-00786-9