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Apolipophorin-II/I Contributes to Cuticular Hydrocarbon Transport and Cuticle Barrier Construction in Locusta migratoria.
- Source :
- Frontiers in Physiology; 7/8/2020, Vol. 11, p1-14, 14p
- Publication Year :
- 2020
-
Abstract
- Apolipophorins are carrier proteins that bind lipids and mediate their transport from tissue to tissue in animals. Apolipophorin I and II (apoLp-II/I) are the major apolipophorins in insects. The implication of apoLp-II/I in cuticle lipid-barrier formation in insects has not been addressed to date. In the present study, we investigated the function of apoLp-II/I in the migratory locust Locusta migratoria (LmapoLp-II/I). During the development of fifth instar nymphs, LmapoLp-II/I transcript levels increased until mid-instar, and then decreased gradually until molting to the adult stage. We found that LmapoLp-II/I was predominately expressed in the fat body and the integument including oenocytes and epidermal cells. Immunodetection experiments revealed that LmapoLp-I mainly localized in the cytoplasm of oenocytes and epidermal cells. Silencing of LmapoLp-II/I caused molting defects in nymphs. Importantly, RNA interference against LmapoLp-II/I resulted in a significant decrease in the content of cuticle surface lipids including alkanes and methyl alkanes. Cuticular permeability was significantly enhanced in these nymphs in Eosin Y penetration assays. By consequence, desiccation resistance and insecticide tolerance of ds LmapoLp-II/I -treated locusts were reduced. Taken together, our results indicate that LmapoLp-II/I is involved in the transport and deposition of surface-cuticular lipids that are crucial for maintaining normal cuticle barrier function in L. migratoria. [ABSTRACT FROM AUTHOR]
- Subjects :
- MIGRATORY locust
CUTICLE
CARRIER proteins
FAT
INSECTICIDE resistance
Subjects
Details
- Language :
- English
- ISSN :
- 1664042X
- Volume :
- 11
- Database :
- Complementary Index
- Journal :
- Frontiers in Physiology
- Publication Type :
- Academic Journal
- Accession number :
- 144461051
- Full Text :
- https://doi.org/10.3389/fphys.2020.00790