Changes in the expression of vasoconstrictors in cerebrospinal fluid from patients with subarachnoid hemorrhage.

Background Cerebral vasospasm (CVS) is one of the common severe complications in patients with subarachnoid hemorrhage (SAH), as well as an independent risk factor for poor prognosis. As previous studies showed, CVS is closely related to the changes in the expression of vasoconstrictors in cerebrospinal fluid (CSF). In the present study, we measured the levels of endothelin-1 (ET-1), angiotensin II (Ang II) and thromboxane B₂ (TXB₂) in SAH patients' CSF to demonstrate the relationship with CVS. Methods A total of 18 patients with SAH were enrolled from September 2018 to January 2019. CSF samples were collected within 3 d, 3-5 d, 5-7 d, 7-10 d and more than 10 d after SAH. The levels of ET-1, Ang II and TXB2 were detected by enzyme-linked immune sorbent assay (ELISA). Results The levels of Ang II (P = 0.041) and TXB₂ (P = 0.050) in CSF at different time points were significantly different. The level of Ang II at ≥ 10 d after SAH was significantly lower than that at < 3 d (P = 0.009) and 5-7 d (P = 0.000) after SAH. The level of TXB₂ reached the peak at 5-7 d after SAH (P = 0.036, 0.008), and then gradually decreased without significance. The level of ET - 1 in CSF at different time points were not different (P > 0.05). Conclusions The levels of Ang II and TXB₂ in SAH patients' CSF present dynamic variation with the progression of the disease. This may be involved in the occurrence of CVS. [ABSTRACT FROM AUTHOR]