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Different Intensity Exercise Preconditions Affect Cardiac Function of Exhausted Rats through Regulating TXNIP/TRX/NF-ĸBp65/NLRP3 Inflammatory Pathways.
- Source :
- Evidence-based Complementary & Alternative Medicine (eCAM); 6/9/2020, p1-9, 9p, 1 Color Photograph, 2 Charts, 3 Graphs
- Publication Year :
- 2020
-
Abstract
- Objective. To investigate whether exercise preconditioning (EP) improves the rat cardiac dysfunction induced by exhaustive exercise (EE) through regulating NOD-like receptor protein 3 (NLRP3) inflammatory pathways and to confirm which intensity of EP is better. Method. Ninety healthy male Sprague Dawley rats were randomly divided into five groups: a control group (CON), exhaustive exercise group (EE), low-, middle-, and high-intensity exercise precondition and exhaustive exercise group (LEP + EE, MEP + EE, HEP + EE group). We established the experimental model by referring to Bedford's motion load standard to complete the experiment. Then, the pathological changes of the myocardium were observed under a light microscope. Biomarker of myocardial injury in serum and oxidative stress factor in myocardial tissue were evaluated by ELISAs. The cardiac function parameters were detected using a Millar pressure and volume catheter. The levels of thioredoxin-interacting protein (TXNIP), thioredoxin protein (TRX), nuclear transcription factor kappa B<subscript>p65</subscript> (NF-ĸB<subscript>p65</subscript>), NLRP3, and cysteinaspartate specific proteinase 1 (Caspase-1) protein in rats' myocardium were detected by western blotting. Results. 1. The myocardial structures of three EP + EE groups were all improved compared with EE groups. 2. The levels of the creatine phosphating-enzyme MB (CK-MB), reactive oxygen species (ROS), interleukin-6 (IL-6), C-reactive protein (CRP), and tumor necrosis factor alpha (TNF-α) in three EP + EE groups were all increased compared with CON but decreased compared with the EE group (P < 0.05). 3. Compared with the CON group, slope of end-systolic pressure volume relationship (ESPVR), ejection fraction (EF), and peak rate of the increase in pressure (dP/dt<subscript>max</subscript>) all dropped to the lowest level in the EE group (P < 0.05), while the values of cardiac output (CO), stroke volume (SV), end-systolic volume (Ves), end-diastolic volume (Ved), and relaxation time constant (Tau) increased in the EE group (P < 0.05). 4. Compared with the CON group, the expression levels of TXNIP, NF-ĸB<subscript>p65</subscript>, NLRP3, and Caspase-1 all increased obviously in the other groups (P < 0.05); meanwhile, they were all decreased in three EP + EE groups compared with the EE group (P < 0.05). 5. NLRP3 was positively correlated with heart rate, IL-6, and ROS, but negatively correlated with EF (P < 0.01). Conclusion. EP protects the heart from EE-induced injury through downregulating TXNIP/TRX/NF-ĸB<subscript>p65</subscript>/NLRP3 inflammatory signaling pathways. Moderate intensity EP has the best protective effect. [ABSTRACT FROM AUTHOR]
- Subjects :
- REACTIVE oxygen species
ANIMAL experimentation
BIOMARKERS
BLOOD pressure
C-reactive protein
CARDIAC output
CARDIOVASCULAR diseases risk factors
CELLULAR signal transduction
EXERCISE therapy
EXPERIMENTAL design
HEART rate monitoring
INFLAMMATORY mediators
INTERLEUKINS
MYOCARDIUM
PEPTIDES
PHOSPHOCREATINE
PROTEIN kinases
RATS
TUMOR necrosis factors
WESTERN immunoblotting
DNA-binding proteins
OXIDATIVE stress
EXERCISE intensity
CASPASES
SIGNAL peptides
STROKE volume (Cardiac output)
VENTRICULAR ejection fraction
Subjects
Details
- Language :
- English
- ISSN :
- 1741427X
- Database :
- Complementary Index
- Journal :
- Evidence-based Complementary & Alternative Medicine (eCAM)
- Publication Type :
- Academic Journal
- Accession number :
- 143683620
- Full Text :
- https://doi.org/10.1155/2020/5809298