Optimization of Supersaturated Solution from Stevia rebaudiana Water Extract Lead to Crystal Nucleation.

The nucleation of crystal growth is thought influenced by supersaturated solution. Crystallization of steviol glycoside based on the water extract of Stevia rebaudiana is challenging. We use factorial 4³D-optimal design for simultaneously optimizing supersaturation solution. As factors applied were concentrating the solution such as the addition of antisolvent, temperature and time in crystal formation. Each factor consisted of 3 levels. The supersaturated solution was observed by measurement of total dissolved solids and total suspended solids. Stevioside and rebaudioside A concentration were determined from solution which obtained in optimum condition by High Performance Liquid Chromatography (HPLC). The optimal condition of supersaturated solution was reached at a concentration of 5%, the addition of 90% ethanol as an antisolvent, a temperature of 5 °C, and a period of crystal growth for 6 hours. Optimization of supersaturation from the water extract of S. rebaudiana lead to the formation of heterogeneous primary nuclei of the crystallization process. [ABSTRACT FROM AUTHOR]