Antimicrobial Potency from Endophytic Bacteria of Bignay Plant (Antidesma bunius (L.) Spreng.) Against Pathogenic Bacteria.

The need for drugs is increasing in the antibiotic field. Synthetic antibiotics consist some chemicals and also has a bad impact on body health, so that the use of endophytic bacteria asa an antibiotic source is carried out The purpose of this research is to knowing the antibacterial potency of bignay's plant endophytic bacteria against pathogenic bacteria, that is Gram-negative bacteria i.e Salmonella thypimurium ATCC 14028, Escherichia coli ATCC 35218 and Gram-positive bacteria i.e Staphyllococcus aureus ATCC 6538. This research used descriptive method, that consist of endopyhtic bacterial isolation, identification, and antimicrobial test. Endopyhtic bacterial isolation from fruit and stem of Bignay plant is performed by using pour plate method. Identification of endophytic bacteria is performed by biochemical test method which is using Vitek 2.0. Antimicrobial test is performed by using paper disc diffusion method. The result is endophytic bacteria isolated from fruit and stem of Bignay plant is Bacillus pumilus, Bacillus amyloliquefaciens, Bacillus cereus, Bacillus sp.1, and Bacillus sp.2. The most potential endophytic bacteria as antimicrobial against pathogenic bacteria is Bacillus pumilus, Bacillus sp.1, and Bacillus sp.2. [ABSTRACT FROM AUTHOR]