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Matrix-assisted laser desorption ionisation (MALDI) mass spectrometry (MS): basics and clinical applications.

Authors :
Israr, Muhammad Zubair
Bernieh, Dennis
Salzano, Andrea
Cassambai, Shabana
Yazaki, Yoshiyuki
Suzuki, Toru
Source :
Clinical Chemistry & Laboratory Medicine; Jun2020, Vol. 58 Issue 6, p883-896, 14p, 1 Diagram, 2 Charts
Publication Year :
2020

Abstract

Background: Matrix-assisted laser desorption ionisation (MALDI) mass spectrometry (MS) has been used for more than 30 years. Compared with other analytical techniques, it offers ease of use, high throughput, robustness, cost-effectiveness, rapid analysis and sensitivity. As advantages, current clinical techniques (e.g. immunoassays) are unable to directly measure the biomarker; rather, they measure secondary signals. MALDI-MS has been extensively researched for clinical applications, and it is set for a breakthrough as a routine tool for clinical diagnostics. Content: This review reports on the principles of MALDI-MS and discusses current clinical applications and the future clinical prospects for MALDI-MS. Furthermore, the review assesses the limitations currently experienced in clinical assays, the advantages and the impact of MALDI-MS to transform clinical laboratories. Summary: MALDI-MS is widely used in clinical microbiology for the screening of microbial isolates; however, there is scope to apply MALDI-MS in the diagnosis, prognosis, therapeutic drug monitoring and biopsy imaging in many diseases. Outlook: There is considerable potential for MALDI-MS in clinic as a tool for screening, profiling and imaging because of its high sensitivity and specificity over alternative techniques. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14346621
Volume :
58
Issue :
6
Database :
Complementary Index
Journal :
Clinical Chemistry & Laboratory Medicine
Publication Type :
Academic Journal
Accession number :
143112942
Full Text :
https://doi.org/10.1515/cclm-2019-0868