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Smartphone-based multiplex 30-minute nucleic acid test of live virus from nasal swab extract.

Authors :
Sun, Fu
Ganguli, Anurup
Nguyen, Judy
Brisbin, Ryan
Shanmugam, Krithika
Hirschberg, David L.
Wheeler, Matthew B.
Bashir, Rashid
Nash, David M.
Cunningham, Brian T.
Source :
Lab on a Chip; 5/7/2020, Vol. 20 Issue 9, p1621-1627, 7p
Publication Year :
2020

Abstract

Rapid, sensitive and specific detection and reporting of infectious pathogens is important for patient management and epidemic surveillance. We demonstrated a point-of-care system integrated with a smartphone for detecting live virus from nasal swab media, using a panel of equine respiratory infectious diseases as a model system for corresponding human diseases such as COVID-19. Specific nucleic acid sequences of five pathogens were amplified by loop-mediated isothermal amplification on a microfluidic chip and detected at the end of reactions by the smartphone. Pathogen-spiked horse nasal swab samples were correctly diagnosed using our system, with a limit of detection comparable to that of the traditional lab-based test, polymerase chain reaction, with results achieved in ∼30 minutes. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14730197
Volume :
20
Issue :
9
Database :
Complementary Index
Journal :
Lab on a Chip
Publication Type :
Academic Journal
Accession number :
143082750
Full Text :
https://doi.org/10.1039/d0lc00304b