MiR-377 Mediates the Effect of Syk on Atherosclerosis in ApoE-/- Mice.

Objective: To study the effect of MiR-377-mediated Syk on atherosclerosis in ApoE/mice. Methods: The experimental subjects were divided into control group (ApoE_/mice), MiR- 377 group (ApoE_/mice injected with MiR-377), Syk group (ApoE_/mice injected with Syk) and Syk+ MiR-377 group (ApoE_/mice injected with MiR-377and Syk). MiR-377 singlestranded antisense nucleotide drugs and Syk gene sequences were prepared and transfected into ApoE_/mice. The expression of Syk gene and protein in ApoE_/mice was detected by qPCR and Western blot, and the levels of inflammatory factors, lipid and oxidative dismutase were detected. The effects of MiR-377 on atherosclerotic aortic plaque and surface area in ApoE_/mice were studied by HE staining and oil red O staining. Results: The relative expression of Syk gene in MiR-377 group was significantly lower than that in control group, and the expression of Syk protein in MiR-377 group was significantly lower than that in control group. TNF-alpha levels in Syk group were significantly lower than those in control group and MiR-377 group. The 1L-6 level in the MiR-377 group was significantly higher than that in the control group, while the 1L-6 level in the Syk group and the MiR377+Syk group was significantly lower than that in the control group. The level of 1L-1ß in the MiR377 group was significantly lower than that in the control group. The TC level of MiR-377 group was significantly lower than that of control group, and the 1L-1ß level of Syk group was significantly higher than that of control group and MiR-377 group. The level of LDL in the MiR-377 group was significantly lower than that in the control group, the level of HDL in the MiR-377 group was significantly lower than that in the control group, and the level of HDL in the Syk group was significantly higher than that in the control group and the MiR-377 group. The level of SOD in the MiR-377 group was significantly higher than that in the control group. There were obvious aortic plaques in the control group and Syk group. There were no obvious aortic plaques in the MiR-377+Syk group and the MiR-377 treated group. The area of atherosclerotic plaque in ApoE_/mice treated with MiR-377 decreased significantly. The plaque area of ApoE-/- mice treated with Syk increased. The ratio of sclerotic plaque area to total area was 25.63% in control group, 7.81% in mi-377 group, 35.19% in Syk group and 9.73% in Syk + MiR-377 group. Conclusion: Overexpression of Syk gene can increase lipid accumulation and oxidative stress in atherosclerotic cells. Mir-377 can regulate the development of atherosclerosis by inhibiting the expression of Syk gene. [ABSTRACT FROM AUTHOR]