AMELIORATIVE EFFECT OF COMMERCIAL GREEN TEA ON IBUPROFEN INDUCED HISTOMORPHOLOGICAL ALTERATION IN LUMINAL DIAMETER OF PROXIMAL RENAL TUBULE.

Objective: To study the effects of commercial green tea on ibuprofen induced effects on luminal diameter of proximal renal tubule in adult rat. Study Design: Laboratory based experimental study. Place and Duration of Study: Army Medical College Rawalpindi and National Institute of Health (NIH) Islamabad, from Mar 2017 to May 2017. Methodology: Thirty healthy adult Sprague Dawley rats were divided into three groups, each containing 10 animals. Schedule of intervention was once a day for a period of 8 weeks. Group Awascontrol. Group B received only ibuprofen 120 mg/kg bodyweight once daily. Group C was given green tea 1ml/100 mg bodyweight in addition to ibuprofen 120mg/kg bodyweight, once a day. At the end of 8 weeks, all animals were sacrificed and kidneys were dissected out. Renal tissue was then prepared for histological study. Luminal diameters of proximal renal tubules were measured. Results: Luminal diameter of proximal renal tubule was found significantly reduced (4.79 ± 0.92μm) in experimental group B (ibuprofen only), as compared to those in control group A (13.073 ± 1.02μm) and in experimental group C (12.67 ± 1.34μm). However, the reduction in proximal luminal diameter in group C was not significant as compared to group A. Conclusion: Ibuprofen caused reduction in proximal luminal diameter in kidneys of adult rats. However, administration of green tea along with ibuprofen protects against the adverse effects of the drug. [ABSTRACT FROM AUTHOR]