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Feasibility of the Bag-Mediated Filtration System for Environmental Surveillance of Poliovirus in Kenya.

Authors :
Zhou, Nicolette A.
Fagnant-Sperati, Christine S.
Komen, Evans
Mwangi, Benlick
Mukubi, Johnstone
Nyangao, James
Hassan, Joanne
Chepkurui, Agnes
Maina, Caroline
van Zyl, Walda B.
Matsapola, Peter N.
Wolfaardt, Marianne
Ngwana, Fhatuwani B.
Jeffries-Miles, Stacey
Coulliette-Salmond, Angela
PeƱaranda, Silvia
Shirai, Jeffry H.
Kossik, Alexandra L.
Beck, Nicola K.
Wilmouth, Robyn
Source :
Food & Environmental Virology; Mar2020, Vol. 12 Issue 1, p35-47, 13p
Publication Year :
2020

Abstract

The bag-mediated filtration system (BMFS) was developed to facilitate poliovirus (PV) environmental surveillance, a supplement to acute flaccid paralysis surveillance in PV eradication efforts. From April to September 2015, environmental samples were collected from four sites in Nairobi, Kenya, and processed using two collection/concentration methodologies: BMFS (> 3 L filtered) and grab sample (1 L collected; 0.5 L concentrated) with two-phase separation. BMFS and two-phase samples were analyzed for PV by the standard World Health Organization poliovirus isolation algorithm followed by intratypic differentiation. BMFS samples were also analyzed by a cell culture independent real-time reverse transcription polymerase chain reaction (rRT-PCR) and an alternative cell culture method (integrated cell culture-rRT-PCR with PLC/PRF/5, L20B, and BGM cell lines). Sabin polioviruses were detected in a majority of samples using BMFS (37/42) and two-phase separation (32/42). There was statistically more frequent detection of Sabin-like PV type 3 in samples concentrated with BMFS (22/42) than by two-phase separation (14/42, p = 0.035), possibly due to greater effective volume assayed (870 mL vs. 150 mL). Despite this effective volume assayed, there was no statistical difference in Sabin-like PV type 1 and Sabin-like PV type 2 detection between these methods (9/42 vs. 8/42, p = 0.80 and 27/42 vs. 32/42, p = 0.18, respectively). This study demonstrated that BMFS can be used for PV environmental surveillance and established a feasible study design for future research. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
18670334
Volume :
12
Issue :
1
Database :
Complementary Index
Journal :
Food & Environmental Virology
Publication Type :
Academic Journal
Accession number :
142041116
Full Text :
https://doi.org/10.1007/s12560-019-09412-1