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Signal-off electrochemiluminescence immunosensors based on the quenching effect between curcumin-conjugated Au nanoparticles encapsulated in ZIF-8 and CdS-decorated TiO2 nanobelts for insulin detection.
- Source :
- Analyst; 3/7/2020, Vol. 145 Issue 5, p1858-1864, 7p
- Publication Year :
- 2020
-
Abstract
- A new strategy for the highly sensitive electrochemiluminescence (ECL) detection of insulin was developed based on curcumin-conjugated Au nanoparticles wrapped in zeolitic Zn<superscript>2+</superscript>-imidazolate cross-linked framework nanoparticles (Au-Cur/ZIF-8) quenching the ECL of CdS-decorated TiO<subscript>2</subscript> nanobelts (CdS@TiO<subscript>2</subscript>). Curcumin-conjugated Au nanoparticles were embedded in ZIF-8 through epitaxial growth or nucleus coalescence. Herein, dual coreactants H<subscript>2</subscript>O<subscript>2</subscript> and K<subscript>2</subscript>S<subscript>2</subscript>O<subscript>8</subscript> were employed to enhance the ECL emission of CdS@TiO<subscript>2</subscript>, and the enhancing mechanism may be due to the electrogenerated hydroxyl radical (OH˙) strengthening the amount of sulfate radical anion (SO<subscript>4</subscript>˙<superscript>−</superscript>), which was the main species of generating ECL signals. In order to sensitively detect insulin, Au-Cur/ZIF-8 labels were used to anchor secondary antibodies via amidation reaction and inhibit the ECL intensity of CdS@TiO<subscript>2</subscript>. Moreover, the possible mechanism of quenching between Au-Cur/ZIF-8 and CdS@TiO<subscript>2</subscript> was ascribed to the poor conductivity of ZIF-8, the consumption of OH˙ and the ECL resonance energy transfer between CdS@TiO<subscript>2</subscript> and Au-Cur. Under optimal conditions, a sandwich immunosensor was prepared to determine insulin with a good linear range from 0.3 pg mL<superscript>−1</superscript> to 20 ng mL<superscript>−1</superscript> and a low detection limit of 0.09 pg mL<superscript>−1</superscript> (S/N = 3). [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00032654
- Volume :
- 145
- Issue :
- 5
- Database :
- Complementary Index
- Journal :
- Analyst
- Publication Type :
- Academic Journal
- Accession number :
- 142022429
- Full Text :
- https://doi.org/10.1039/c9an02288k