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Role of carbohydrate moieties in cross-reactivity between different components of <em>Parietaria judaica</em> pollen extract.
- Source :
- Allergy; Aug92 Part 2, Vol. 47, p424-430, 7p
- Publication Year :
- 1992
-
Abstract
- Cross-reactivity between the different components in <em>Parietaria judaica</em> pollen extract has been investigated by polyclonal as well as monoclonal antibodies before, and after chemical deglycosylation obtained by trifluoromethanesulphonic acid (TFMS) treatment of the extract. In western blotting a polyclonal rabbit antiserum, obtained by injecting purified Par j I, was able to recognise many components of the native extract. However, its reactivity was restricted, after chemical deglycosylation of the extract, to the major allergen alone, indicating that its cross-reactivity was due to sugar moieties. Moreover, out of several monoclonal antibodies raised by injecting the whole <em>Parietaria judaica</em> extract, one (1A4/2F8) was also able in western blotting to recognise an epitope shared by many components of the extract except the major allergen Par j I. However, in this case the broad reactivity of the antibody was not affected by the deglycosylating procedure. When the reactivity of <em>Parietaria judaica</em> extract was tested before and after sugar removal, against specific IgE from a pool of patient sera, no differences could be demonstrated, thus indicating that carbohydrates are not strongly involved in the binding of <em>Parietaria judaica</em>-specific IgE. The results indicate that both proteic and carbohydratic cross-reactive epitopes are shared by many components of <em>Parietaria judaica</em> pollen extract. [ABSTRACT FROM AUTHOR]
- Subjects :
- CARBOHYDRATES
MOIETIES (Chemistry)
POLLEN
SERUM
ALLERGENS
PATIENTS
Subjects
Details
- Language :
- English
- ISSN :
- 01054538
- Volume :
- 47
- Database :
- Complementary Index
- Journal :
- Allergy
- Publication Type :
- Academic Journal
- Accession number :
- 14198062
- Full Text :
- https://doi.org/10.1111/j.1398-9995.1992.tb02083.x