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Role of carbohydrate moieties in cross-reactivity between different components of <em>Parietaria judaica</em> pollen extract.

Authors :
Mucci, N.
Liberatore, P.
Federico, R.
Forlani, F.
Di Felice, G.
Afferni, C.
Tinghino, R.
De Cesare, F.
Pini, C.
Source :
Allergy; Aug92 Part 2, Vol. 47, p424-430, 7p
Publication Year :
1992

Abstract

Cross-reactivity between the different components in &lt;em&gt;Parietaria judaica&lt;/em&gt; pollen extract has been investigated by polyclonal as well as monoclonal antibodies before, and after chemical deglycosylation obtained by trifluoromethanesulphonic acid (TFMS) treatment of the extract. In western blotting a polyclonal rabbit antiserum, obtained by injecting purified Par j I, was able to recognise many components of the native extract. However, its reactivity was restricted, after chemical deglycosylation of the extract, to the major allergen alone, indicating that its cross-reactivity was due to sugar moieties. Moreover, out of several monoclonal antibodies raised by injecting the whole &lt;em&gt;Parietaria judaica&lt;/em&gt; extract, one (1A4/2F8) was also able in western blotting to recognise an epitope shared by many components of the extract except the major allergen Par j I. However, in this case the broad reactivity of the antibody was not affected by the deglycosylating procedure. When the reactivity of &lt;em&gt;Parietaria judaica&lt;/em&gt; extract was tested before and after sugar removal, against specific IgE from a pool of patient sera, no differences could be demonstrated, thus indicating that carbohydrates are not strongly involved in the binding of &lt;em&gt;Parietaria judaica&lt;/em&gt;-specific IgE. The results indicate that both proteic and carbohydratic cross-reactive epitopes are shared by many components of &lt;em&gt;Parietaria judaica&lt;/em&gt; pollen extract. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
01054538
Volume :
47
Database :
Complementary Index
Journal :
Allergy
Publication Type :
Academic Journal
Accession number :
14198062
Full Text :
https://doi.org/10.1111/j.1398-9995.1992.tb02083.x