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Genome-wide gene expression profiling of the melon fly, Zeugodacus cucurbitae, during thirteen life stages.
- Source :
- Scientific Data; 2/11/2020, Vol. 7 Issue 1, p1-9, 9p
- Publication Year :
- 2020
-
Abstract
- The melon fly, Zeugodacus cucurbitae (Coquillett), is an important destructive pest worldwide. Functional studies of the genes associated with development and reproduction during different life stages are limited in Z. cucurbitae. There have yet to be comprehensive transcriptomic resources for genetic and functional genomic studies to identify the molecular mechanisms related to its development and reproduction. In this study, we comprehensively sequenced the transcriptomes of four different developmental stages: egg, larva, pupa, and adults. Using the Illumina RNA-Seq technology, we constructed 52 libraries from 13 stages with four biological replicates in each and generated 435.61 Gb clean reads. We comprehensively characterized the transcriptomes with high-coverage mapping to the reference genome. A total of 13,760 genes were mapped to the reference genome, and another 4481 genes were characterized as new genes. Finally, 14,931 genes (81.85%) were functionally annotated against six annotation databases. This study provides the first comprehensive transcriptome data of all developmental stages of Z. cucurbitae, and will serve as a valuable resource for future genetic and functional studies. Measurement(s) RNA • sequence_assembly • sequence feature annotation • gene expression Technology Type(s) RNA sequencing • sequence assembly process • sequence annotation • Expectation-Maximization(EM) algorithm Factor Type(s) biological replicate • developmental stage • sex Sample Characteristic - Organism Zeugodacus cucurbitae Machine-accessible metadata file describing the reported data: 10.6084/m9.figshare.11698254 [ABSTRACT FROM AUTHOR]
- Subjects :
- GENOMES
GENE expression
MELON fly
LARVAE
TRANSCRIPTOMES
Subjects
Details
- Language :
- English
- ISSN :
- 20524463
- Volume :
- 7
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Scientific Data
- Publication Type :
- Academic Journal
- Accession number :
- 141680873
- Full Text :
- https://doi.org/10.1038/s41597-020-0387-9