Ultrasensitive immunosensor for AFP detection based on Cu2O to generate electrical signals.

In this experiment, a Cu2O spherical nanoparticle was reduced by hydrothermal reaction to prepare a label-free electrochemical immunosensor based on a novel signal amplification strategy. Multi-walled carbon nanotubes are a platform that combines cuprous oxide with carbon nanotubes. Nanogold was modified on the surface of cuprous oxide nanospheres by electrostatic adsorption to further amplify the electrochemical signal and link-supported anti-alpha-fetoprotein (AFP). Under optimal conditions, the proposed immunosensor has a wide linear range from 0.001 to 40 ng/ml, with a detection limit as low as 0.33 pg/ml (S/N = 3). Furthermore, the proposed label-free immunosensor has been used to determine AFP in human serum with satisfactory results. At the same time, it has good reproducibility, acceptable selectivity and long-term stability, and has broad application prospects in biometric analysis. [ABSTRACT FROM AUTHOR]