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Structural and biophysical properties of RIG-I bound to dsRNA with G-U wobble base pairs.
- Source :
- RNA Biology; Mar2020, Vol. 17 Issue 3, p325-334, 10p
- Publication Year :
- 2020
-
Abstract
- Retinoic acid-inducible gene I (RIG-I) is responsible for innate immunity via the recognition of short double-stranded RNAs in the cytosol. With the clue that G-U wobble base pairs in the influenza A virus's RNA promoter region are responsible for RIG-I activation, we determined the complex structure of RIG-I ΔCARD and a short hairpin RNA with G-U wobble base pairs by X-ray crystallography. Interestingly, the overall helical backbone trace was not affected by the presence of the wobble base pairs; however, the base pair inclination and helical axis angle changed upon RIG-I binding. NMR spectroscopy revealed that RIG-I binding renders the flexible base pair of the influenza A virus's RNA promoter region between the two G-U wobble base pairs even more flexible. Binding to RNA with wobble base pairs resulted in a more flexible RIG-I complex. This flexible complex formation correlates with the entropy-favoured binding of RIG-I and RNA, which results in tighter binding affinity and RIG-I activation. This study suggests that the structure and dynamics of RIG-I are tailored to the binding of specific RNA sequences with different flexibility. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 15476286
- Volume :
- 17
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- RNA Biology
- Publication Type :
- Academic Journal
- Accession number :
- 141626857
- Full Text :
- https://doi.org/10.1080/15476286.2019.1700034