The Analysis about the Changes of Gut Microbiome after 8-Week Administration of Proton Pump Inhibitor.

Background/Aims Although the long-term administration of proton pump inhibitors (PPIs) has been proven to induce the changes of gut microbiota, study about the short-term effect of PPIs is lacking. We aimed to verify the influence of 8 weeks of PPI treatment on the gut microbiome. Methods Patients who were given 8 weeks of Ilaprazole (20 mg, once daily PO) in the treatment of gastroesophageal reflux diseases were prospectively enrolled in our study. Enrolled subjects were educated to avoid changes in eating habits and other medications. The fecal samples were collected for microbiome analysis before and right after PPI administration. The microbial 16S rDNA of the fecal sample was collected, amplified and analyzed by the polymerase chain reaction method and sequenced using the Illumina MiSeq® System. Microbial information was obtained using the NCBI 16S database and we analyzed the variety of species and the variation of the microbiota. Results Nine patients were finally analyzed in our study. There was no significant change in species diversity when compared before and after PPI administration. The results of classifying gut microbiota in the colony as genus showed increases in Streptococcus, Agathobaculum, and Murimonas. As for species, eight species were significantly increased (such as Streptococcus), and one species, Sutterella wadsworthensis was decreased after PPI administration. When gut microbiota was classified as genus and species, 12 genus and 28 species showed statistical differences in their composition fraction. Conclusions The short-term (8 weeks) administration of Ilaprazole were found to affect the abundance and composition ratio of gut microbiome at the level of a strain, although there was no significant change in the cluster diversity of gut microbiome. [ABSTRACT FROM AUTHOR]