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MicroRNA-7, synergizes with RORα, negatively controls the pathology of brain tissue inflammation.
- Source :
- Journal of Neuroinflammation; 1/20/2020, Vol. 17 Issue 1, p1-19, 19p
- Publication Year :
- 2020
-
Abstract
- <bold>Background: </bold>Accumulating evidence has documented that microRNA-7 (miR-7) plays an important role in the pathology of various diseases. However, the potential role of miR-7 in brain tissue inflammation (BTI) remains unclear.<bold>Methods: </bold>We detected the expression of miR-7 in LPS-induced murine BTI model and observed the possible effects of miR-7 deficiency on the pathology of BTI. To elucidate the mechanism, the target gene of miR-7 was screened out by Gene chip assay and its potential roles in BTI were evaluated by Western blot, immunofluorescence, and RNAi assay, respectively.<bold>Results: </bold>MiR-7 was upregulated in brain tissue in BTI mice and its deficiency could significantly aggravate the pathology of brain tissue. Moreover, RORα, a new target molecule of miR-7, was upregulated in brain tissue from miR-7 deficiency BTI mice. Of note, downregulation of RORα could remarkably exacerbate the pathology of brain tissue and elevate the transduction of NF-κB and ERK1/2 signaling pathways in brain tissue from miR-7 deficiency BTI mice. Furthermore, RORα and miR-7 were dominantly co-expressed in neurons of BTI mice. Finally, RORα synergized with miR-7 to control the inflammatory reaction of neuronal cells in response to LPS stimulation.<bold>Conclusions: </bold>MiR-7 expression is upregulated in BTI model. Moreover, miR-7 synergizes with its target gene RORα to control the inflammation reaction of neurons, thereby orchestrating the pathology of BTI. [ABSTRACT FROM AUTHOR]
- Subjects :
- ENCEPHALITIS
BRAIN diseases
BRAINWASHING
PATHOLOGY
WESTERN immunoblotting
Subjects
Details
- Language :
- English
- ISSN :
- 17422094
- Volume :
- 17
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Neuroinflammation
- Publication Type :
- Academic Journal
- Accession number :
- 141291645
- Full Text :
- https://doi.org/10.1186/s12974-020-1710-2