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Late-maturity α-amylase expression in wheat is influenced by genotype, temperature and stage of grain development.
- Source :
- Planta: An International Journal of Plant Biology; Feb2020, Vol. 251 Issue 2, p1-14, 14p
- Publication Year :
- 2020
-
Abstract
- Main Conclusion: Late-maturity α-amylase (LMA) expression in wheat grains can be induced by either a cool temperature shock close to physiological maturity or continuous cool maximum temperatures during grain development. Late-maturity α-amylase (LMA) is a genetic trait in wheat (Triticum aestivum L.) involving the production of α-amylase during grain development, which can result in an unacceptably low Falling Number (FN) in mature grain and consequent grain downgrading. Comparison of the FN test, an α-amylase activity assay and a high pI α-amylase-specific ELISA on the same meal samples gave equivalent results; ELISA was used for further experiments because of its isoform specificity. A cool temperature shock during the middle stages of grain development is known to induce LMA and is used for phenotypic screening. It was determined that a cool temperature treatment of seven days was required to reliably induce LMA. Glasshouse studies performed in summer and winter demonstrated that temperature affected the timing of sensitivity to cool-shock by altering the rate and duration of grain development, but that the sensitive grain developmental stage was unchanged at 35–45% moisture content. Wheat varieties with Rht-B1b or Rht-D1b dwarfing genes responded to a cool-shock only from mid grain filling until physiological maturity, whilst genotypes with Rht8c or without a dwarfing gene expressed LMA in response to a cool-shock during a wider developmental range. A continuous cool maximum temperature regimen (23 °C/15 °C day/night) during grain development also resulted in LMA expression and showed a stronger association with field expression than the cool-shock treatment. These results clarify how genotype, temperature and grain developmental stage determine LMA expression, and allow for the improvement of LMA phenotypic screening methods. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00320935
- Volume :
- 251
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Planta: An International Journal of Plant Biology
- Publication Type :
- Academic Journal
- Accession number :
- 141244240
- Full Text :
- https://doi.org/10.1007/s00425-020-03341-1