STUDY OF GENETIC VARIATION OF NADH DEHYDROGENASE SUBUNIT 5 (NAD5) GENE FOR HYMENOLEPIS NANA ISOLATED FROM DIFFERENT HOST (MICE AND RATS) IN DIYALA PROVINCE, IRAQ.

Hymenolepis nana (H. nana), the dwarf tapeworm, is the smaller and widespread tapeworm among humans people all over the world. Current study aimed to detect the genetic variation of nad5 gene for H. nana between isolated samples from different hosts (mice and rats) and phylogenetic relationships between sequences them. Using polymerase chain reaction (PCR) and parts of nad5 gene was amplified by PCR and sequenced directly. Data were analysed by using (bioedit v 7.0 Software) for alignment of sequences and (MEGA6 software) for UPGMA analysis and dendrograms phylogenetic tree to isolates of H. nana from different hosts compared with Genbank published isolates of H. nana; (KT589891.1; KT589905.1; KT951722.1; AP017666.1, KT589901.1). Results showed nucleotide substitutions in positions 261 from G- A in sample 9, 10 of H. nana isolates from rat and nucleotide substitutions in positions 280 from G-A in all sample of H. nana isolates from rat and mice compare with reference sequence of Gene Bank nad5. Regarding in phylogenetic analysis, our data similarity with 100% with Genbank published excepted minimum similarity (99.9%) with (KT589901.1). However, there is not obvious differences between of H. nana mice and rat isolates are seen in cluster of phylogenetic tree. [ABSTRACT FROM AUTHOR]