Plasma pseudouridine levels reflect body size in children on hemodialysis.

Background: Dialysis in children as well as adults is prescribed to achieve a target spKt/V_urea, where V_urea is the volume of distribution of urea. Waste solute production may however be more closely correlated with body surface area (BSA) than V_urea which rises in proportion with body weight. Plasma levels of waste solutes may thus be higher in smaller patients when targeting spKt/V_urea since they have higher BSA relative to body weight. This study measured levels of pseudouridine (PU), a novel marker solute whose production is closely proportional to BSA, to test whether prescription of dialysis to a target spKt/V_urea results in higher plasma levels of PU in smaller children. Methods: PU and urea nitrogen (ureaN) were measured in plasma and dialysate at the midweek hemodialysis session in 20 pediatric patients, with BSA ranging from 0.65–1.87m². Mathematical modeling was employed to estimate solute production rates and average plasma solute levels. Results: The dialytic clearance (K_d) of PU was proportional to that of ureaN (average K_dPU/K_dUreaN 0.69 ± 0.13, r² 0.84, p < 0.001). Production of PU rose in proportion with BSA (r² 0.57, p < 0.001). The pretreatment plasma level of PU was significantly higher in smaller children (r² 0.20, p = 0.051) while the pretreatment level of ureaN did not vary with size. Conclusions: Prescribing dialysis based on urea kinetics may leave uremic solutes at higher levels in small children. Measurement of a solute produced proportional to BSA may provide a better index of dialysis adequacy than measurement of urea. [ABSTRACT FROM AUTHOR]