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Epitope specificity of IgE antibodies to a major allergen (Cry j 1) of Japanese cedar pollen in sera of humans and monkeys with pollinosis.
Epitope specificity of IgE antibodies to a major allergen (Cry j 1) of Japanese cedar pollen in sera of humans and monkeys with pollinosis.
- Source :
- Immunology; Jun97, Vol. 91 Issue 2, p161-166, 6p
- Publication Year :
- 1997
-
Abstract
- Japanese cedar (Cryptomeria japonica) pollinosis has been reported to occur naturally in Japanese monkeys (Macaca fuscata) as well as humans. Using monoclonal antibodies (mAb) specific to Cry j 1, a major allergen in Japanese cedar pollen, we identified five independent epitopes (EPA to EP-5) on the molecule. The epitopes recognized by IgE antibodies in the sera of humans and monkeys with the pollinosis were analysed by an IgE enzyme-linked immunosorbent assay inhibition method with these mAb. In human patients, the mAb to EPA strongly blocked the binding of IgE antibodies in all patients' sera to Cry j 1. The reaction patterns of IgE antibodies in monkeys, however, varied among the troops of monkeys. In some troops, the mAb to EPA showed a blocking pattern similar to that for human patients. In other troops, mAb to EP-4 and EP-5 blocked binding of IgE. These results indicate that some, but not all, monkeys have antibody responses to the major allergen similar to those of humans. [ABSTRACT FROM AUTHOR]
- Subjects :
- CEDAR
JAPANESE macaque
CRYPTOMERIA japonica
CRYPTOMERIA
ALLERGENS
IMMUNOGLOBULIN G
Subjects
Details
- Language :
- English
- ISSN :
- 00192805
- Volume :
- 91
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- Immunology
- Publication Type :
- Academic Journal
- Accession number :
- 14068751
- Full Text :
- https://doi.org/10.1046/j.1365-2567.1997.00255.x