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Epitope specificity of IgE antibodies to a major allergen (Cry j 1) of Japanese cedar pollen in sera of humans and monkeys with pollinosis.

Epitope specificity of IgE antibodies to a major allergen (Cry j 1) of Japanese cedar pollen in sera of humans and monkeys with pollinosis.

Authors :
Sakaguchi, M.
Hashimoto, M.
Nigi, H.
Yasueda, H.
Takahashi, Y.
Watanabe, M.
Nagoya, T.
Taniguchi, Y.
Kurmoto, M.
Inouye, S.
Source :
Immunology; Jun97, Vol. 91 Issue 2, p161-166, 6p
Publication Year :
1997

Abstract

Japanese cedar (Cryptomeria japonica) pollinosis has been reported to occur naturally in Japanese monkeys (Macaca fuscata) as well as humans. Using monoclonal antibodies (mAb) specific to Cry j 1, a major allergen in Japanese cedar pollen, we identified five independent epitopes (EPA to EP-5) on the molecule. The epitopes recognized by IgE antibodies in the sera of humans and monkeys with the pollinosis were analysed by an IgE enzyme-linked immunosorbent assay inhibition method with these mAb. In human patients, the mAb to EPA strongly blocked the binding of IgE antibodies in all patients' sera to Cry j 1. The reaction patterns of IgE antibodies in monkeys, however, varied among the troops of monkeys. In some troops, the mAb to EPA showed a blocking pattern similar to that for human patients. In other troops, mAb to EP-4 and EP-5 blocked binding of IgE. These results indicate that some, but not all, monkeys have antibody responses to the major allergen similar to those of humans. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
00192805
Volume :
91
Issue :
2
Database :
Complementary Index
Journal :
Immunology
Publication Type :
Academic Journal
Accession number :
14068751
Full Text :
https://doi.org/10.1046/j.1365-2567.1997.00255.x