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First Reported Nosocomial Outbreak Of NDM-5-Producing Klebsiella pneumoniae In A Neonatal Unit In China.
- Source :
- Infection & Drug Resistance; Nov2019, Vol. 12, p3557-3566, 10p
- Publication Year :
- 2019
-
Abstract
- Purpose: Carbapenem-resistant Klebsiella pneumoniae (CRKP) have emerged worldwide and also being a major threat to children and neonate. In this study, we describe a nosocomial outbreak of NDM-5-producing Klebsiella pneumoniae in neonatal unit of a teaching hospital in China from September 2015 to September 2016. Patients and methods: We collected 12 carbapenem-resistant K. pneumoniae outbreak strains from 12 newborns and characterized these isolates for their antimicrobial susceptibility, clone relationships, and multi-locus sequence types using vitek-2 compact system, pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Resistant genes were detected by using PCR and sequencing. Plasmid conjugation experiment was carried out to determine the transferability of carbapenem resistance. PCR-based replicon typing (PBRT), S1 nuclease-PFGE, and southern blotting were conducted for plasmid profiling. Results: All 12 K. pneumoniae isolates were resistant to carbapenems and carried bla<subscript>NDM-5</subscript>, bla<subscript>TEM-1</subscript> and bla<subscript>SHV-11</subscript>. Furthermore, PFGE analysis showed that NDM-5-producing K. pneumoniae were clonally related and MLST assigned them to sequence type 337. Conjugative assays showed that plasmids harboring bla<subscript>NDM-5</subscript> gene were self-transmissible. Plasmid analysis suggested that all bla<subscript>NDM-5</subscript> gene located on a ∼45 kb IncX3 type plasmid. Conclusion: To the best of our knowledge, this is the first report of a clone outbreak of bla<subscript>NDM-5</subscript>-carrying K. pneumoniae isolates from neonates. There is an urgent need for effective infection control measures to prevent bla<subscript>NDM-5</subscript> variants from becoming epidemic in the neonates in the future. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 11786973
- Volume :
- 12
- Database :
- Complementary Index
- Journal :
- Infection & Drug Resistance
- Publication Type :
- Academic Journal
- Accession number :
- 140403349
- Full Text :
- https://doi.org/10.2147/IDR.S218945