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Effective fraction of Bletilla striata reduces the inflammatory cytokine production induced by water and organic extracts of airborne fine particulate matter (PM2.5) in vitro.
- Source :
- BMC Complementary & Alternative Medicine; 12/16/2019, Vol. 19 Issue 1, p1-12, 12p, 1 Diagram, 2 Charts, 9 Graphs
- Publication Year :
- 2019
-
Abstract
- Background: Bletilla striata is a traditional Chinese medicine used to treat hemorrhage, scald, gastric ulcer, pulmonary diseases and inflammations. In this study, we investigated bioactivity of the effective fraction of B. striata (EFB) in reducing the inflammatory cytokine production induced by water or organic extracts of PM<subscript>2.5</subscript>. Methods: PM<subscript>2.5</subscript> extracts were collected and analyzed by chromatographic system and inductively coupled plasma mass spectrometer. Cell viability was measured using MTS (3-(4,5-dimethylthiazol-2-yl)-5-(3-carboxymethoxyphenyl)-2-(4-sulfophenyl)-2H-tetrazolium) assay, and cell supernatant was analyzed by flow cytometry, ELISA, and qRT-PCR in cultured mouse macrophage cell line RAW264.7 treated with EFB and PM<subscript>2.5</subscript> extracts. Expressions of nuclear factor-kappa B (NF-κB) and mitogen-activated protein kinase (MAPK) signaling pathway were measured by Western blot. Results: PM<subscript>2.5</subscript> composition is complex and the toxicity of PM<subscript>2.5</subscript> extracts were not noticeable. The treatment of EFB at a wide dose-range of 0–40 μg/mL did not cause significant change of RAW264.7 cell proliferation. EFB pretreatment decreased the inflammatory cytokines in the macrophage. Further analysis showed that EFB significantly attenuated PM<subscript>2.5</subscript>-induced proinflammatory protein expression and downregulated the levels of phosphorylated NF-κBp65, inhibitor of kappa B (IκB)-α, c-Jun N-terminal kinase (JNK), extracellular signal-regulated kinase (ERK), and p38. Conclusions: Our study demonstrated the potential effectiveness of B. striata extracts for treating PM<subscript>2.5</subscript>-triggered pulmonary inflammation. [ABSTRACT FROM AUTHOR]
- Subjects :
- INFLAMMATION prevention
CELL proliferation
ANIMAL experimentation
BIOLOGICAL assay
CELL lines
CELLULAR signal transduction
CHROMATOGRAPHIC analysis
COLORIMETRY
CYTOKINES
ENZYME-linked immunosorbent assay
FLOW cytometry
GENE expression
HERBAL medicine
INFLAMMATION
LUNGS
MACROPHAGES
MASS spectrometry
CHINESE medicine
MICE
PHOSPHORYLATION
POLYMERASE chain reaction
PROTEIN kinases
WESTERN immunoblotting
DNA-binding proteins
PARTICULATE matter
REVERSE transcriptase polymerase chain reaction
CELL survival
IN vitro studies
DRUG administration
DRUG dosage
Subjects
Details
- Language :
- English
- ISSN :
- 14726882
- Volume :
- 19
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- BMC Complementary & Alternative Medicine
- Publication Type :
- Academic Journal
- Accession number :
- 140371197
- Full Text :
- https://doi.org/10.1186/s12906-019-2790-3