Effects of Toll-like receptor 4 antagonist Eritrane on neurogenesis and gamma-aminobutyric acid glutamate balance in prefrontal lobe and hippocampus of depressive rats.

Objective: To investigate the effects of Toll-like receptor 4 antagonist Eritrane on neurogenesis and gamma-aminobutyric acid glutamate balance in the prefrontal lobe and hippocampus of depressive rats. Methods: 100 healthy SD rats were divided into healthy control group, blank control group and Eritram group (low dose group, medium dose group and high dose group), 20 rats in each group. The depression model of rats in blank control group and Salvia miltiorrhiza group was established. After the completion of the model, rats in the blank control group were injected with normal saline and rats in the Eritrean group were injected with high, medium and low doses of Eritrean injection respectively. After 21 days of continuous administration, the total distance of spontaneous activity, the immobility time of forced swimming, the levels of gamma-aminobutyric glutamate (GABA), glutamate (Glu) and Tolllike receptor 4 (TLR-4) protein in prefrontal lobe and hippocampal neurons were measured and compared. Pearson correlation test was used to analyze the correlation between TLR4 and GABA, Glu levels in depressive rats. Results: Compared with the healthy control group, the total spontaneous activity distance of the blank control group and Eritrean group decreased significantly, and the immobility time of forced swimming increased significantly (P<0.05). There was a significant difference between the blank control group and Eritrean group (P<0.05). The rats in Eritrean group spontaneously survived with the increase of dose. The total distance increased, while the immobility time of forced swimming decreased (P<0.05). Compared with the healthy control group, the levels of GABA in the prefrontal lobe and hippocampus of rats in blank control group and Eritrean group decreased significantly, the levels of Glu and TLR4 increased significantly, and the levels of GABA in Eritrean group were higher than those in blank control group, and the levels of Glu and TLR4 were lower than those in blank control group. TLR4 and TLR4 decreased significantly (P<0.05). Pearson correlation test showed that TLR4 was negatively correlated with GABA and positively correlated with Glu (P<0.05). Conclusion: Eritrean can reduce the effects of depression on the neurogenesis of prefrontal lobe and hippocampal neurons and the balance of gamma-aminobutyric acid and glutamate in rats. The mechanism is that Eritrean can increase the level of GABA and decrease the level of Glu by antagonizing Toll-like receptor 4, thus exerting the neuroprotective effect of prefrontal lobe and hippocampal neurons. [ABSTRACT FROM AUTHOR]