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Upregulation of VEGF and PEDF in Placentas of Women with Lower Extremity Venous Insufficiency during Pregnancy and Its Implication in Villous Calcification.
- Source :
- BioMed Research International; 12/9/2019, p1-8, 8p
- Publication Year :
- 2019
-
Abstract
- Pregnancy is a period in a woman's life in which changes can occur that affect different physiological processes. Common conditions during this period include vascular changes, such as lower extremity venous insufficiency (VI). This is an observational, analytical, and prospective cohort study in which 114 pregnant women were analyzed, of which 62 were clinically diagnosed with VI. In parallel, 52 control patients without VI (HC) were studied. The aim of this study was to observe changes in angiogenesis and inflammation markers as well as the presence of calcium deposits. The expression of vascular endothelial growth factor (VEGF), transforming growth factor-β (TGF-β), and pigment epithelium-derived factor (PEDF) was analyzed by immunohistochemistry and RT-qPCR. The presence of calcium deposits was revealed using the von Kossa method. In the placentas of mothers with VI, gene expression of VEGF (34.575 [32.380–36.720] VI vs 32.965 [30.580–36.320] HC) and PEDF (25.417 [24.459–27.675] VI vs 24.400 [23.102–30.223] HC) significantly increased, as was protein expression in the placental villi. An increase in calcium deposits was observed in the placentas of women with VI (72.58% VI/53.84% HC). This study revealed the existence of cellular damage in the placental villi of mothers with VI with tissue implications such as increased calcification. [ABSTRACT FROM AUTHOR]
- Subjects :
- CHORIONIC villi
GENE expression
IMMUNOHISTOCHEMISTRY
LEG
LONGITUDINAL method
SCIENTIFIC observation
PLACENTA
POLYMERASE chain reaction
PREGNANT women
RESEARCH
TRANSFORMING growth factors-beta
VENOUS insufficiency
PROTEASE inhibitors
VASCULAR endothelial growth factors
REVERSE transcriptase polymerase chain reaction
CALCINOSIS
DISEASE complications
DISEASE risk factors
PREGNANCY
Subjects
Details
- Language :
- English
- ISSN :
- 23146133
- Database :
- Complementary Index
- Journal :
- BioMed Research International
- Publication Type :
- Academic Journal
- Accession number :
- 140267483
- Full Text :
- https://doi.org/10.1155/2019/5320902