Back to Search
Start Over
Defining mesenchymal stromal cell (MSC)-derived small extracellular vesicles for therapeutic applications.
- Source :
- Journal of Extracellular Vesicles; Dec2019, Vol. 8 Issue 1, p1-10, 10p
- Publication Year :
- 2019
-
Abstract
- Small extracellular vesicles (sEVs) from mesenchymal stromal/stem cells (MSCs) are transiting rapidly towards clinical applications. However, discrepancies and controversies about the biology, functions, and potency of MSC-sEVs have arisen due to several factors: the diversity of MSCs and their preparation; various methods of sEV production and separation; a lack of standardized quality assurance assays; and limited reproducibility of in vitro and in vivo functional assays. To address these issues, members of four societies (SOCRATES, ISEV, ISCT and ISBT) propose specific harmonization criteria for MSC-sEVs to facilitate data sharing and comparison, which should help to advance the field towards clinical applications. Specifically, MSC-sEVs should be defined by quantifiable metrics to identify the cellular origin of the sEVs in a preparation, presence of lipid-membrane vesicles, and the degree of physical and biochemical integrity of the vesicles. For practical purposes, new MSC-sEV preparations might also be measured against a well-characterized MSC-sEV biological reference. The ultimate goal of developing these metrics is to map aspects of MSC-sEV biology and therapeutic potency onto quantifiable features of each preparation. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 20013078
- Volume :
- 8
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Journal of Extracellular Vesicles
- Publication Type :
- Academic Journal
- Accession number :
- 140252605
- Full Text :
- https://doi.org/10.1080/20013078.2019.1609206