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Ex vivo perfusion-based engraftment of genetically engineered cell sensors into transplantable organs.

Authors :
Chin, Ling-Yee
Carroll, Cailah
Raigani, Siavash
Detelich, Danielle M.
Tessier, Shannon N.
Wojtkiewicz, Gregory R.
Schmidt, Stephen P.
Weissleder, Ralph
Yeh, Heidi
Uygun, Korkut
Parekkadan, Biju
Source :
PLoS ONE; 12/2/2019, Vol. 14 Issue 12, p1-14, 14p
Publication Year :
2019

Abstract

Cellular rejection of liver transplant allografts remains a concern despite immunosuppressant use. Existing transplant biomarkers are often not sensitive enough to detect acute or chronic rejection at an early enough stage to allow successful clinical intervention. We herein developed a cell-based sensor that can potentially be used for monitoring local events following liver transplantation. Utilizing a machine perfusion system as a platform to engraft the cells into a donor liver, we effectively established the biocompatibility of the biosensor cells and confirmed efficient delivery of cells distributed throughout the organ. This work proves an innovative concept of integrating synthetic reporter cells ex vivo into organs as a transplant-within-a-transplant during functional organ preservation with a vision to use cell biosensors as a broad way to monitor and treat tissue transplants. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
19326203
Volume :
14
Issue :
12
Database :
Complementary Index
Journal :
PLoS ONE
Publication Type :
Academic Journal
Accession number :
140030809
Full Text :
https://doi.org/10.1371/journal.pone.0225222