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C3N4 nanosheet-supported Prussian Blue nanoparticles as a peroxidase mimic: colorimetric enzymatic determination of lactate.
- Source :
- Microchimica Acta; Nov2019, Vol. 186 Issue 11, pN.PAG-N.PAG, 1p
- Publication Year :
- 2019
-
Abstract
- Prussian Blue nanoparticles were deposited on g-C<subscript>3</subscript>N<subscript>4</subscript> nanosheets. The resulting nanocomposite possesses peroxidase-like (POx) activity and can catalyze the oxidation of 3,3′,5,5′-tetramethylbenzidine at room temperature in the presence of H<subscript>2</subscript>O<subscript>2</subscript>. This leads to formation of a blue product with an absorption maximum at 650 nm. The formation of the Prussian Blue nanoparticles on the g-C<subscript>3</subscript>N<subscript>4</subscript> nanosheets, and the POx-like activity of the composite were characterized in detail. The POx mimic was used for determination of L-lactic acid via detection of H<subscript>2</subscript>O<subscript>2</subscript> that is produced by the enzyme lactate oxidase (LOx). The assay has a linear range that extends from 5 to 100 μM, and the detection limit is 2.2 μM. The method was successfully applied to the determination of L-lactic acid in spiked human serum. [ABSTRACT FROM AUTHOR]
- Subjects :
- PEROXIDASE
PRUSSIAN blue
HORSERADISH peroxidase
DETECTION limit
GALENA
LACTATES
Subjects
Details
- Language :
- English
- ISSN :
- 00263672
- Volume :
- 186
- Issue :
- 11
- Database :
- Complementary Index
- Journal :
- Microchimica Acta
- Publication Type :
- Academic Journal
- Accession number :
- 139744341
- Full Text :
- https://doi.org/10.1007/s00604-019-3834-6