Back to Search
Start Over
The evaluation of anoxia responsive E2F DNA binding activity in the red eared slider turtle, Trachemys scripta elegans.
- Source :
- PeerJ; May2019, p1-15, 15p
- Publication Year :
- 2019
-
Abstract
- In many cases, the DNA-binding activity of a transcription factor does not change, while its transcriptional activity is greatly influenced by the make-up of bound proteins. In this study, we assessed the protein composition and DNA-binding ability of the E2F transcription factor complex to provide insight into cell cycle control in an anoxia tolerant turtle through the use of a modified ELISA protocol. This modification also permits the use of custom DNA probes that are tailored to a specific DNA binding region, introducing the ability to design capture probes for non-model organisms. Through the use of EMSA and ELISA DNA binding assays, we have successfully determined the in vitro DNA binding activity and complex dynamics of the Rb/E2F cell cycle regulatory mechanisms in an anoxic turtle, Trachemys scripta elegans. Repressive cell cycle proteins (E2F4, Rb, HDAC4 and Suv39H1) were found to significantly increase at E2F DNA-binding sites upon anoxic exposure in anoxic turtle liver. The lack of p130 involvement in the E2F DNA-bound complex indicates that anoxic turtle liver may maintain G<subscript>1</subscript> arrest for the duration of stress survival. [ABSTRACT FROM AUTHOR]
- Subjects :
- LOGGERHEAD turtle
TURTLES
DNA
BINDING site assay
DNA probes
TRANSCRIPTION factors
Subjects
Details
- Language :
- English
- ISSN :
- 21678359
- Database :
- Complementary Index
- Journal :
- PeerJ
- Publication Type :
- Academic Journal
- Accession number :
- 139657726
- Full Text :
- https://doi.org/10.7717/peerj.4755