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LMNA-mutated Rabbits: A Model of Premature Aging Syndrome with Muscular Dystrophy and Dilated Cardiomyopathy.

Authors :
Tingting Sui
Di Liu
Tingjun Liu
Jichao Deng
Mao Chen
Yuanyuan Xu
Yuning Song
Hongsheng Ouyang
Liangxue Lai
Zhanjun Li
Source :
Aging & Disease; Jan2018, Vol. 10 Issue 1, p102-115, 14p
Publication Year :
2019

Abstract

Premature aging syndromes are rare genetic disorders mimicking clinical and molecular features of aging. Products of the LMNA gene, primarily lamin A and C, are major components of the nuclear lamina. A recently identified group of premature aging syndromes was related to mutations of the LMNA gene. Although LMNA disorders have been identified in premature aging syndromes, affect specifically the skeletal muscles, cardiac muscles, and lipodystrophy, understanding the pathogenic mechanisms still need to be elucidated. Here, to establish a rabbit knockout (KO) model of premature aging syndromes, we performed precise LMNA targeting in rabbits via co-injection of Cas9/sgRNA mRNA into zygotes. The LMNA-KO rabbits exhibited reduced locomotion activity with abnormal stiff walking posture and a shortened stature, all of them died within 22 days. In addition, cardiomyopathy, muscular dystrophy, bone and joint abnormalities, as well as lipodystrophy were observed in LMNA-KO rabbits. In conclusion, the novel rabbit LMNA-KO model, displayed typical features of histopathological defects that are observed in premature aging syndromes, and may be utilized as a valuable resource for understanding the pathophysiological mechanisms of premature aging syndromes and elucidating mysteries of the normal process of aging in humans. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
21525250
Volume :
10
Issue :
1
Database :
Complementary Index
Journal :
Aging & Disease
Publication Type :
Academic Journal
Accession number :
139020916
Full Text :
https://doi.org/10.14336/AD.2018.0209