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Analysis of the lipoteichoic-acid-like macroamphiphile from <em>Bifidobacterium bifidum</em> subspecies <em>Pennsylvanicum</em> by one- and two-dimensional 1H- and 13C-NMR spectroscopy.
- Source :
- European Journal of Biochemistry; 6/15/87, Vol. 165 Issue 3, p647-652, 6p, 5 Diagrams, 2 Charts
- Publication Year :
- 1987
-
Abstract
- The native lipoteichoic-acid-like amphiphite from <em>Bifidobacterium bifidum</em> subspecies pennsylvanicum and its basic glycan moiety, obtained by alkaline hydrolysis, were studied by <superscript>1</superscript>H-, <superscript>13</superscript>C-, and <superscript>31</superscript>P-NMR, DEPT (dis- torsionless enhancement by polarization transfer), <superscript>1</superscript>H-<superscript>1</superscript>H correlation spectroscopy and <superscript>13</superscript>C-<superscript>1</superscript>H shift correlation NMR spectroscopy. The results are consistent with the structure elucidated by chemical analysis (Fischer, W., preceding paper in this journal): ... and establish the structures of the repeating units independently. In addition to characteristic shifts in the <superscript>13</superscript>C-NMR spectrum, the sites of phosphodiester and alanine ester bonds are also manifest in the <superscript>1</superscript>H-NMR spectrum. Substitution of the glycerophosphate residues can be recognized in the <superscript>31</superscript>P-NMR spectrum also. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 165
- Issue :
- 3
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13891246
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1987.tb11489.x