Effect of pretreatment of polydatin on cell proliferation of renal podocytes MPC5 cells of mice induced by TGF-β1.

Objective To observe the effect of polydatin on the proliferation of renal cell line MPC5 induced by TGF-β₁ in mice, and to explore its possible mechanism. Methods MPC5 cells were divided into four groups: groups A, B, C, and D. The cells in the groups A, B and C were pretreated with polydatin 100 μmol/L for 24 h. After pretreatment, cells in the group A were cultured with polydatin for 2 h, and two hours later, 10 ng/mL TGF-β₁ were added in cells for 24 h. In the group B, 30 μmol/L of the inhibitor of miR-21 was added, and six hours later, 100 μmol/L polydatin were added, for 2 h, followed by addition of TGF-β₁ for culturing 24 h. In the group C, 10 ng/mL TGF-β₁ were added for culturing 24 h. However, the group D was regarded as the blank control group, and it was added the ordinary culture medium. At the end of the training, the cell proliferation [optical density (OD value J was determined by MTT, the cell oxidative stress indicators superoxide dismutase (SOD) and malondialdehyde (MDA) content was detected by spectrophotometer, the reactive oxygen species (ROS) content was detected by 2', 7 '-dichloro dihydrogen fluorescein diacetate ester load method, and the miR-21 and Nod-like receptor pyrin containing 3 (NLRP3), caspase-1, interleukin 1β (IL-1β) protein were determined by Western blotting. Results The OD values in the groups A, B, C and D were 14. 73 ± l. 60, 12. 70 ± l. 13, 19. 31 ± l. 79 and 12. 62 ± l. 26, respectively. Compared with group D, the OD value increased in the group C (P < 0. 05); compared with group C, the OD value decreased in the group A (P < 0. 05); compared with group B, the OD value of the group A increased (P <0. 05). At 24 h after culture, compared with group D, the relative expression levels of MDA, ROS, miR-21, NLRP3, caspase-1 and IL-1β in the group C all increased, while the relative expression level of SOD decreased (all P < 0. 05). Compared with the group C, the relative expression levels of MDA, ROS, miR-21, NLRP3, caspase-1 and IL-1β in the group A all decreased, while the relative expression level of SOD increased (all P < 0. 05). Compared with the group B, the relative expression levels of MDA, ROS, miR-21, NLRP3, caspase-1 and IL-1 β in the group A increased, while the relative expression of SOD decreased (all P < 0. 05). Conclusions Polydatin can inhibit the proliferation of MPC5 cells induced by TGF-β₁. The possible mechanism is that polydatin regulates miR-21-related signaling pathways, further increases SOD content, reduces intracellular MDA and ROS content, and inhibits the expression of NLRP3, caspase-1 and IL-1β proteins. [ABSTRACT FROM AUTHOR]