Back to Search
Start Over
Oral administration of oat beta-glucan preparations of different molecular weight results in regulation of genes connected with immune response in peripheral blood of rats with LPS-induced enteritis.
- Source :
- European Journal of Nutrition; Oct2019, Vol. 58 Issue 7, p2859-2873, 15p, 1 Diagram, 6 Charts, 1 Graph
- Publication Year :
- 2019
-
Abstract
- Purpose: Beta-glucans are biologically active polysaccharides having antioxidant, immunomodulatory, and antiinflammatory properties. This study investigated the transcriptomic profile in peripheral blood of rats with LPS-induced enteritis, which were fed a diet supplemented with high- (G1) and low- (G2) molecular-weight oat beta-glucans. Methods: Two-color rat gene expression microarrays were applied and the analysis was performed using a common reference design to provide easy means of comparing samples from various experimental conditions against one another. Common reference sample was labeled with cyanine 3 (Cy3) and investigated samples from each experimental group: C-G0 (control group fed semi-synthetic diet), LPS-G0 (LPS-challenged group fed semi-synthetic diet), LPS-G1 (LPS-challenged group fed G1 beta-glucan enriched diet), and LPS-G2 (LPS-challenged group fed G2 beta-glucan enriched diet) were labeled with cyanine 5 (Cy5). Each microarray was performed in quadruplicate. Statistical analysis was performed using one-way ANOVA and Tukey's HSD post-hoc test (p < 0.05). A multiple testing correction was performed using Benjamini and Hochberg False Discovery Rate < 5%. A quantitative real-time RT-PCR was performed to verify the expression of chosen transcripts. Results: The microarray analyses revealed differentially expressed transcripts between: the LPS-G0 and the control groups: C-G0 (138 genes), the LPS-G1 and LPS-G0 groups (533 genes), and the LPS-G2 and LPS-G0 groups (97 genes). Several differentially expressed genes in the beta-glucan-supplemented groups encoded proteins belonging to TLR and NLR signaling pathways, as well as prostaglandin synthesis and regulation pathways. Both beta-glucans up-regulated the expression of Atg10, which belongs to the family of autophagy-related genes, suggesting a possible link between autophagy induction and beta-glucan supplementation. Conclusion: The changes in gene expression observed in the peripheral blood indicate that oat beta-glucans exerted a protective effect in rats with an induced inflammatory state caused by LPS challenge. The greater number of differentially expressed genes was observed in group supplemented with G1 beta-glucan, pointing at the differences in the mode of action of high- and low-molecular-weight beta-glucans in the organism. [ABSTRACT FROM AUTHOR]
- Subjects :
- AUTOPHAGY
ANIMAL experimentation
BLOOD
CELLULAR signal transduction
PHYSICAL & theoretical chemistry
COMPARATIVE studies
DIETARY supplements
ELEMENTAL diet
ENTERITIS
GENE expression
GENES
HYDROCARBONS
OATS
ORAL drug administration
POLYMERASE chain reaction
PROSTAGLANDINS
PROTEINS
RATS
STATISTICS
THIAZOLES
DATA analysis
TREATMENT effectiveness
LIPOPOLYSACCHARIDES
GENE expression profiling
TOLL-like receptors
SIGNAL peptides
DESCRIPTIVE statistics
BETA-glucans
ONE-way analysis of variance
PHARMACODYNAMICS
Subjects
Details
- Language :
- English
- ISSN :
- 14366207
- Volume :
- 58
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- European Journal of Nutrition
- Publication Type :
- Academic Journal
- Accession number :
- 138885590
- Full Text :
- https://doi.org/10.1007/s00394-018-1838-3