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The accessibility of 5-methylcytosine to specific antibodies in double-stranded DNA of <em>Xanthomonas</em> phage XP12.
- Source :
- European Journal of Biochemistry; 10/1/85, Vol. 152 Issue 1, p115-121, 7p
- Publication Year :
- 1985
-
Abstract
- Antibodies specifically directed to 5-methylcytidine were raised in rabbits and purified by affinity chromatography. The accessibility of 5-methyldeoxycytidine (m<superscript>5</superscript>dCyd) to such antibodies was studied with DNAs from various origins. The reaction was followed by measuring the retention of radiolabelled DNA by antibodies on nitrocellulose filters, by immunoprecipitation, by gel filtration and was visualized with the electron microscope. Antibodies did not bind to Escherichia coli B DNA, which is deficient in m<superscript>5</superscript>dCyd. Denatured and native DNA from calf thymus, which contains m<superscript>5</superscript>dCyd as a minor nucleoside, was weakly retained on the filters whereas DNA extracted from Xanthomonas oryzac XP12 bacteriophage, which is rich in m<superscript>5</superscript>dCyd, was well recognized even in the native form. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 152
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13873478
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1985.tb09170.x