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Study of the conversion of GDP-mannose into GCP-fucose in Nereids: a biochemical marker of oocyte maturation.
- Source :
- European Journal of Biochemistry; 10/15/84, Vol. 144 Issue 2, p255-259, 5p
- Publication Year :
- 1984
-
Abstract
- Homogenates of Perinereis cultrifera oocytes were found to transform GDP-D-mannose into another sugar nucleotide. Ultraviolet absorption spectra, chromatographic behaviour, gas-liquid chromatography coupled to mass spectrometry analysis revealed that GDP-D-mannose had been converted into GDP-L-fucose. This conversion is a multi-step reaction as proved by the involvement of two intermediates identified as GDP-4-oxo-6-deoxy-D-mannose and GDP-4-oxo-6-deoxy-L-galactose, this latter being reduced by NADPH to give GDP-L-fucose. It is shown that the enzymatic activities responsible for the conversion of GDP-D-mannose into GDP-L-fucose is recovered only in oocytes and is not present in the other coclomic cells (i.e. coelomocytes). More interesting is the fact that maximum activity is recovered at a well defined stage of the hormone-controlled oogenesis. Thus, this enzymatic system appears as a biochemical marker of the oocyte maturation in P. cultrifera. [ABSTRACT FROM AUTHOR]
- Subjects :
- CHROMATOGRAPHIC analysis
BIOMARKERS
BIOCHEMISTRY
MANNOSE
GALACTOSE
CELLS
Subjects
Details
- Language :
- English
- ISSN :
- 00142956
- Volume :
- 144
- Issue :
- 2
- Database :
- Complementary Index
- Journal :
- European Journal of Biochemistry
- Publication Type :
- Academic Journal
- Accession number :
- 13853066
- Full Text :
- https://doi.org/10.1111/j.1432-1033.1984.tb08458.x