Diversification of the downy mildew resistance gene pool by introgression of a new gene, Pl35, from wild Helianthus argophyllus into oilseed and confection sunflowers (Helianthus annuus L.).

Key message: We have mapped a new downy mildew resistance gene, Pl₃₅, derived from wild Helianthus argophyllus to sunflower linkage group 1. New germplasms incorporating the Pl₃₅ gene were developed for both oilseed and confection sunflower Sunflower downy mildew (DM), caused by the oomycete pathogen Plasmopara halstedii, is an economically important and widespread sunflower disease worldwide. Non-race-specific resistance is not available in sunflower, and breeding for DM resistance relies on race-specific resistance to control this disease. The discovery of the novel DM resistance genes is a long-term task due to the highly virulent and aggressive nature of the P. halstedii pathogen, which reduces the effectiveness of resistance genes. The objectives of this study were to: (1) transfer DM resistance from a wild sunflower species Helianthus argophyllus (PI 494576) into cultivated sunflowers; (2) map the resistance gene; and (3) develop diagnostic single-nucleotide polymorphism (SNP) markers for efficient targeting of the gene in breeding programs. The H. argophyllus accession PI 494576 previously identified with resistance to the most virulent P. halstedii race 777 was crossed with oilseed and confection sunflower in 2012. Molecular mapping using the BC₂F₂ and BC₂F₃ populations derived from the cross CONFSCLB1/PI 494576 located a new resistance gene Pl₃₅ on linkage group 1 of the sunflower genome. The new gene Pl₃₅ was successfully transferred from PI 494576 into cultivated sunflowers. SNP markers flanking Pl₃₅ were surveyed in a validation panel of 548 diversified sunflower lines collected globally. Eleven SNP markers were found to be diagnostic for Pl₃₅ SNP alleles, with four co-segregating with Pl₃₅. The developed oilseed and confection germplasms with diagnostic SNP markers for Pl₃₅ will be very useful resources for breeding of DM resistance in sunflower. [ABSTRACT FROM AUTHOR]