Back to Search
Start Over
Genome-wide identification of natural RNA aptamers in prokaryotes and eukaryotes.
- Source :
- Nature Communications; 3/29/2018, Vol. 9 Issue 1, p1-10, 10p
- Publication Year :
- 2018
-
Abstract
- RNAs are well-suited to act as cellular sensors that detect and respond to metabolite changes in the environment, due to their ability to fold into complex structures. Here, we introduce a genome-wide strategy called PARCEL that experimentally identifies RNA aptamers in vitro, in a high-throughput manner. By applying PARCEL to a collection of prokaryotic and eukaryotic organisms, we have revealed 58 new RNA aptamers to three key metabolites, greatly expanding the list of natural RNA aptamers. The newly identified RNA aptamers exhibit significant sequence conservation, are highly structured and show an unexpected prevalence in coding regions. We identified a prokaryotic precursor tmRNA that binds vitamin B2 (FMN) to facilitate its maturation, as well as eukaryotic mRNAs that bind and respond to FMN, suggesting FMN as the second RNA-binding ligand to affect eukaryotic expression. PARCEL results show that RNA-based sensing and gene regulation is more widespread than previously appreciated in different organisms. [ABSTRACT FROM AUTHOR]
- Subjects :
- RNA
VITAMIN B2
APTAMERS
GENETIC regulation
PROKARYOTES
METABOLITES
IDENTIFICATION
Subjects
Details
- Language :
- English
- ISSN :
- 20411723
- Volume :
- 9
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Nature Communications
- Publication Type :
- Academic Journal
- Accession number :
- 138017028
- Full Text :
- https://doi.org/10.1038/s41467-018-03675-1