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Optimization of Cellulase and Xylanase Productions by Streptomyces thermocoprophilus Strain TC13W Using Oil Palm Empty Fruit Bunch and Tuna Condensate as Substrates.
- Source :
- Applied Biochemistry & Biotechnology; Sep2019, Vol. 189 Issue 1, p76-86, 11p
- Publication Year :
- 2019
-
Abstract
- The modified medium composed of the alkaline-pretreated oil palm empty fruit bunch (APEFB) and tuna condensate powder was used for cellulase and xylanase productions by Streptomyces thermocoprophilus strain TC13W. The APEFB contained 74.46% (w/w) cellulose, 15.72% (w/w) hemicellulose, and 6.40% (w/w) lignin. The tuna condensate powder contained 55.49% (w/w) protein and 11.05% (w/w) salt. In the modified medium with only 6.75 g/l tuna condensate powder, 10 g/l APEFB, and 0.5 g/l Tween 80, S. thermocoprophilus strain TC13W produced cellulase 4.9 U/ml and xylanase 9.0 U/ml. The enzyme productions in the modified medium were lower than cellulase (6.0 U/ml) and xylanase (12.0 U/ml) productions in the complex medium (CaCl<subscript>2</subscript> 0.1, MgSO<subscript>4</subscript>·7H<subscript>2</subscript>O 0.1, KH<subscript>2</subscript>PO<subscript>4</subscript> 0.5, K<subscript>2</subscript>HPO<subscript>4</subscript> 1.0, NaCl 0.2, yeast extract 5.0, NH<subscript>4</subscript>NO<subscript>3</subscript> 1.0, Tween 80 0.5). When tuna condensate powder in the modified medium was reduced to 5.0 g/l and Tween 80 was increased to 1.5 g/l, S. thermocoprophilus strain TC13W produced cellulase and xylanase activities of 9.1 and 12.1 U/ml, respectively. This study shows that the cost of enzyme production could be reduced by using pretreated EFB and tuna condensate as a carbon and a nitrogen source, respectively. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 02732289
- Volume :
- 189
- Issue :
- 1
- Database :
- Complementary Index
- Journal :
- Applied Biochemistry & Biotechnology
- Publication Type :
- Academic Journal
- Accession number :
- 138011802
- Full Text :
- https://doi.org/10.1007/s12010-019-02986-3