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NF-YC12 is a key multi-functional regulator of accumulation of seed storage substances in rice.
- Source :
- Journal of Experimental Botany; 8/1/2019, Vol. 70 Issue 15, p3765-3780, 16p
- Publication Year :
- 2019
-
Abstract
- Starch and storage proteins, the primary storage substances of cereal endosperm, are a major source of food for humans. However, the transcriptional regulatory networks of the synthesis and accumulation of storage substances remain largely unknown. Here, we identified a rice endosperm-specific gene, NF-YC12 , that encodes a putative nuclear factor-Y transcription factor subunit C. NF-YC12 is expressed in the aleurone layer and starchy endosperm during grain development. Knockout of NF-YC12 significantly decreased grain weight as well as altering starch and protein accumulation and starch granule formation. RNA-sequencing analysis revealed that in the nf-yc12 mutant genes related to starch biosynthesis and the metabolism of energy reserves were enriched in the down-regulated category. In addition, starch and protein contents in seeds differed between NF-YC12 -overexpression lines and the wild-type. NF-YC12 was found to interact with NF-YB1. ChIP-qPCR and yeast one-hybrid assays showed that NF-YC12 regulated the rice sucrose transporter OsSUT1 in coordination with NF-YB1 in the aleurone layer. In addition, NF-YC12 was directly bound to the promoters of FLO6 (FLOURY ENDOSPERM6) and OsGS1;3 (glutamine synthetase1) in developing endosperm. This study demonstrates a transcriptional regulatory network involving NF-YC12, which coordinates multiple pathways to regulate endosperm development and the accumulation of storage substances in rice seeds. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 00220957
- Volume :
- 70
- Issue :
- 15
- Database :
- Complementary Index
- Journal :
- Journal of Experimental Botany
- Publication Type :
- Academic Journal
- Accession number :
- 137997687
- Full Text :
- https://doi.org/10.1093/jxb/erz168