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Optogenetic stimulation of Gs-signaling in the heart with high spatio-temporal precision.

Authors :
Makowka, Philipp
Bruegmann, Tobias
Dusend, Vanessa
Malan, Daniela
Beiert, Thomas
Hesse, Michael
Fleischmann, Bernd K.
Sasse, Philipp
Source :
Nature Communications; 3/20/2019, Vol. 10 Issue 1, p1-9, 9p, 6 Graphs
Publication Year :
2019

Abstract

The standard technique for investigating adrenergic effects on heart function is perfusion with pharmaceutical agonists, which does not provide high temporal or spatial precision. Herein we demonstrate that the light sensitive G<subscript>s</subscript>-protein coupled receptor JellyOp enables optogenetic stimulation of G<subscript>s</subscript>-signaling in cardiomyocytes and the whole heart. Illumination of transgenic embryonic stem cell-derived cardiomyocytes or of the right atrium of mice expressing JellyOp elevates cAMP levels and instantaneously accelerates spontaneous beating rates similar to pharmacological β-adrenergic stimulation. Light application to the dorsal left atrium instead leads to supraventricular extrabeats, indicating adverse effects of localized G<subscript>s</subscript>-signaling. In isolated ventricular cardiomyocytes from JellyOp mice, we find increased Ca<superscript>2+</superscript> currents, fractional cell shortening and relaxation rates after illumination enabling the analysis of differential G<subscript>s</subscript>-signaling with high temporal precision. Thus, JellyOp expression allows localized and time-restricted G<subscript>s</subscript> stimulation and will provide mechanistic insights into different effects of site-specific, long-lasting and pulsatile G<subscript>s</subscript> activation. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
20411723
Volume :
10
Issue :
1
Database :
Complementary Index
Journal :
Nature Communications
Publication Type :
Academic Journal
Accession number :
137987172
Full Text :
https://doi.org/10.1038/s41467-019-09322-7