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Native protein delivery into rice callus using ionic complexes of protein and cell-penetrating peptides.
- Source :
- PLoS ONE; 7/31/2019, Vol. 14 Issue 7, p1-14, 14p
- Publication Year :
- 2019
-
Abstract
- Direct protein delivery into intact plants remains a challenge for the agricultural and plant science fields. Cell-penetrating peptide (CPP)-mediated protein delivery requires the binding of CPPs to a protein to carry the protein into the cell through the cell wall and lipid bilayer. Thus, we prepared ionic complexes of a CPP-containing carrier peptide and a cargo protein, namely, Citrine yellow fluorescent protein, and subsequently studied their physicochemical properties. Two types of carrier peptides, BP100(KH)<subscript>9</subscript> and BP100CH<subscript>7</subscript>, were investigated for delivery efficiency into rice callus. Both BP100(KH)<subscript>9</subscript> and BP100CH<subscript>7</subscript> successfully introduced Citrine protein into rice callus cells under pressure and vacuum treatment. Moreover, delivery efficiency varied at different growth stages of rice callus; 5-day rice callus was a more efficient recipient for Citrine than 21-day callus. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 19326203
- Volume :
- 14
- Issue :
- 7
- Database :
- Complementary Index
- Journal :
- PLoS ONE
- Publication Type :
- Academic Journal
- Accession number :
- 137787930
- Full Text :
- https://doi.org/10.1371/journal.pone.0214033