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PD-L1 Expression with Epithelial Mesenchymal Transition of Circulating Tumor Cells Is Associated with Poor Survival in Curatively Resected Non-Small Cell Lung Cancer.
- Source :
- Cancers; Jun2019, Vol. 11 Issue 6, p806, 1p
- Publication Year :
- 2019
-
Abstract
- In addition to the FDA-approved definition of a circulating tumor cell (CTC), various CTC phenotypes have been discovered. Epithelial-mesenchymal transition (EMT) of cancer cells is directly linked to PD-L1 upregulation. The goal of the study was to investigate PD-L1 expression and EMT in CTCs of non-small cell lung cancer (NSCLC) patients, and perform an outcome analysis. Prospectively, 7.5 mL peripheral blood was collected from 30 NSCLC patients that underwent surgery and 15 healthy controls. CTCs were enriched by size-based microfilter and immunofluorescence stainings performed (cytokeratin (CK) 8/18/19, EpCAM, CD45, PD-L1, EMT markers vimentin, and N-Cadherin, DAPI). Patient-matched NSCLC tissues were also stained. CTC staining intensity was quantified with a software and correlated with patient-matched NSCLC tissues and survival. PD-L1 and EMT markers were expressed at significantly higher proportions in CTCs than patient-matched NSCLC tissues (p < 0.05); ≥3 PD-L1<superscript>pos</superscript>/EMT<superscript>pos</superscript>CTCs were associated with significantly poorer survival after curative surgery (p < 0.05). No CTCs were detected in 15 healthy controls. This study shows that PD-L1 expression and EMT of CTCs is a negative survival predictor for NSCLC patients. The therapeutic role of the molecular linkage of PD-L1 and EMT will need to be further investigated, as linked pathways could be targeted to improve NSCLC outcome. [ABSTRACT FROM AUTHOR]
- Subjects :
- ANTIGEN analysis
EPITHELIAL cells
LUNG cancer diagnosis
LUNG cancer prognosis
GLYCOPROTEIN analysis
CELL adhesion molecules
CELL physiology
CYTOSKELETAL proteins
FLUORESCENT antibody technique
GENE expression
LONGITUDINAL method
LUNG cancer
MEMBRANE proteins
METASTASIS
STAINS & staining (Microscopy)
SURVIVAL
TUMOR markers
FLUORESCENT dyes
Subjects
Details
- Language :
- English
- ISSN :
- 20726694
- Volume :
- 11
- Issue :
- 6
- Database :
- Complementary Index
- Journal :
- Cancers
- Publication Type :
- Academic Journal
- Accession number :
- 137456440
- Full Text :
- https://doi.org/10.3390/cancers11060806