Back to Search Start Over

Different PES nanofibrous membrane parameters effect on the efficacy of immunoassay performance.

Authors :
Mahmoudifard, Matin
Vossoughi, Manuchehr
Source :
Polymers for Advanced Technologies; Aug2019, Vol. 30 Issue 8, p1968-1977, 10p
Publication Year :
2019

Abstract

The contribution of electrospun nanofibrous membranes (e.NFMs) in the biosensing platforms opens up a new prospect for the invention of faster and more sensitive analytical devices. In this paper, we utilized e.NFM of polyethersulfone (PES) as a solid substrate for the protein immobilization through two different approaches: physical and covalent. Scanning electron microscopy (SEM) and Fourier‐transform‐infrared (FTIR) tests were performed to study the effect of plasma treatment on protein immobilization efficacy. Moreover, taking advantage of ELISA technique, the influence of different parameters, namely, nanofibers diameter, membrane thickness, plasma treatment time, an incubation time of ethyl‐3‐(3‐dimethylaminopropyl)‐carbodiimide/N‐hydroxysuccinimide (EDC/NHS), and their ratio on antibody immobilization efficacy through two mentioned approaches, was also assessed. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
10427147
Volume :
30
Issue :
8
Database :
Complementary Index
Journal :
Polymers for Advanced Technologies
Publication Type :
Academic Journal
Accession number :
137376258
Full Text :
https://doi.org/10.1002/pat.4629