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Prevalence, Antibiotic Susceptibility, and Virulence Factors of Yersinia enterocolitica and Related Species from Ready-to-Eat Vegetables Available in Korea.

Prevalence, Antibiotic Susceptibility, and Virulence Factors of Yersinia enterocolitica and Related Species from Ready-to-Eat Vegetables Available in Korea.

Authors :
Tex-Soo Lee
Soon-Won Lee
Won-Seok Seok
Mi-Young Yoo
Jae-Won Yoon
Boo-Kil Park
Kwang-Deog Moon
Deog-Hwan Oh
Source :
Journal of Food Protection; Jun2004, Vol. 67 Issue 6, p1123-1127, 5p, 2 Charts, 1 Graph
Publication Year :
2004

Abstract

A total of 673 ready-to-eat vegetable samples were collected in Korea from 2001 to 2002 and analyzed for the presence of Yersinia spp. We analyzed biotypes, serotypes, and susceptibility to 12 antibiotics and tested for virulence genes of pathogenic Yersinia enterocolitica isolates by PCR assay. Among the samples, 27 (4.0%) were found to be contaminated with Yersinia spp. Among the 27 strains of Yersinia spp. isolates, 18 strains (66.7%) of Y. enterocolitica, 5 strains (18.5%) of Y. frederiksenii, 3 strains (11.1%) of Y. intermedia, and 1 strain (3.7%) of Y. kristensenii were identified. According to the serotypes of Y. enterocolitica isolates, O:3 (11.1%) and O:5 (11.1%) were the most predominant, followed by O:8 (5.6%) and others (72.2%). For biotypes of Y. enterocolitica isolates, 1A (77.8%) was the most predominant, followed by 3B (11.1%), 3 (5.6%), and 5A (5.6%). Also, an antibiotic susceptibility test showed that Y. enterocolitica isolates were very susceptible to the antibiotics tested but highly resistant to ampicillin (94%), cephalothin (100%), and carbenicillin (83%). PCR assays with specific primers derived from yet and ail genes of Y. enterocolitica were applied to confirm the presence of pathogenic Y. enterocolitica. Among the 18 strains of Y. enterocolitica isolates, only 3 strains (O:3/1A, UT/3B, and UT/1A isolated from Chinese cabbage, onion, and spinach, respectively) were shown to have a virulence gene. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
0362028X
Volume :
67
Issue :
6
Database :
Complementary Index
Journal :
Journal of Food Protection
Publication Type :
Academic Journal
Accession number :
13722886
Full Text :
https://doi.org/10.4315/0362-028X-67.6.1123