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Selection of candidate genes affecting meat quality and preliminary exploration of related molecular mechanisms in the Mashen pig.
- Source :
- Asian-Australasian Journal of Animal Sciences; Aug2019, Vol. 32 Issue 8, p1084-1094, 11p
- Publication Year :
- 2019
-
Abstract
- Objective: The aim of this study was to select the candidate genes affecting meat quality and preliminarily explore the related molecular mechanisms in the Mashen pig. Methods: The present study explored genetic factors affecting meat quality in the Mashen pig using RNA sequencing (RNA-Seq). We sequenced the transcriptomes of 180-day-old Mashen and Large White pigs using longissimus dorsi to select differentially expressed genes (DEGs). Results: The results indicated that a total of 425 genes were differentially expressed between Mashen and Large White pigs. A gene ontology enrichment analysis revealed that DEGs were mainly enriched for biological processes associated with metabolism and muscle development, while a Kyoto encyclopedia of genes and genomes analysis showed that DEGs mainly participated in signaling pathways associated with amino acid metabolism, fatty acid metabolism, and skeletal muscle differentiation. A MCODE analysis of the protein-protein interaction network indicated that the four identified subsets of genes were mainly associated with translational initiation, skeletal muscle differentiation, amino acid metabolism, and oxidative phosphorylation pathways. Conclusion: Based on the analysis results, we selected glutamic-oxaloacetic transaminase 1, malate dehydrogenase 1, pyruvate dehydrogenase 1, pyruvate dehydrogenase kinase 4, and activator protein-1 as candidate genes affecting meat quality in pigs. A discussion of the related molecular mechanisms is provided to offer a theoretical basis for future studies on the improvement of meat quality in pigs. [ABSTRACT FROM AUTHOR]
Details
- Language :
- English
- ISSN :
- 10112367
- Volume :
- 32
- Issue :
- 8
- Database :
- Complementary Index
- Journal :
- Asian-Australasian Journal of Animal Sciences
- Publication Type :
- Academic Journal
- Accession number :
- 137103667
- Full Text :
- https://doi.org/10.5713/ajas.18.0718