Biological Activity of New Hydantoin Derivatives on Acinetobacter Baumannii Biofilm Formation Isolated from Clinical Sources.

Wholly 538 clinical samples of blood, burns, sputum, wounds and urine were involved in the current study which was done in 4 hospitals. The isolation and diagnosis of fifty-two Acinetobacter baumannii isolate 36.54% from blood specimens, whereas wounds specimens constituted 23.08%, burns specimens achieved 17.31%, sputum specimens formed 13.46% and a low percentage was in urine specimens which accomplished 9.62%. Totally isolates of bacteria were identified via the biochemical, cultural and microbial characteristics and confirmed via VITEK 2 Compact system. It was found that all (100%) of bacteria isolates were resistant to amoxicillin-clavulanic acid and Cefepime, while Tobramycin was the most effective antibiotic. The isolates displayed the ability of biofilm creation via Microtitration plates method (M.T.P), from the whole number (52) isolates,(33) were producers, such as strong biofilm creators were (15) 45.46% and (18) 54.54% moderate though (19) 36.53% isolates were stately as weak or none biofilm producers. MIC for new hydantoin derivative (C₁₂H₁₃N₃O₂S) was determined against fifteen isolates were active in MIC values equal to 31.25µg/ml gave (7) A. baumannii isolates, while in MIC, values equal to 62.5µg/ml gave (5) isolates, and three isolates were active in MIC values equal to 125µg/ml. [ABSTRACT FROM AUTHOR]