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Genomic analysis of methicillin-resistant Staphylococcus aureus strain SO-1977 from Sudan.

Authors :
Ali, Mohamed S.
Isa, Nurulfiza M.
Abedelrhman, Faisal M.
Alyas, Tahani B.
Mohammed, Sara E.
Ahmed, Abdallah E.
Ahmed, Zainab S. A.
Lau, Nyok-Sean
Garbi, Mohamed I.
Amirul, Abdullah Al-Ashraf
Seed, Almeen O.
Omer, Rihab A.
Mohamed, Sofia B.
Source :
BMC Microbiology; 6/11/2019, Vol. 19 Issue 1, p1-9, 9p
Publication Year :
2019

Abstract

Background: Methicillin-resistant Staphylococcus aureus (MRSA) is known as a leading cause of morbidity and mortality. Investigation of the MRSA's virulence and resistance mechanisms is a continuing concern toward controlling such burdens through using high throughput whole Genome Sequencing (WGS) and molecular diagnostic assays. The objective of the present study is to perform whole-genome sequencing of MRSA isolated from Sudan using Illumina Next Generation Sequencing (NGS) platform. Results: The genome of MRSA strain SO-1977 consists of 2,827,644 bp with 32.8% G + C, 59 RNAs and 2629 predicted coding sequences (CDSs). The genome has 26 systems, one of which is the major class in the disease virulence and defence. A total of 83 genes were annotated to virulence disease and defence category some of these genes coding as functional proteins. Based on genome analysis, it is speculated that the SO-1977 strain has resistant genes to Teicoplanin, Fluoroquinolones, Quinolone, Cephamycins, Tetracycline, Acriflavin and Carbapenems. The results revealed that the SO-1977, strain isolated from Sudan has a wide range of antibiotic resistance compared to related strains. Conclusion: The study reports for the first time the whole genome sequence of Sudan MRSA isolates. The release of the genome sequence of the strain SO-1977 will avail MRSA in public databases for further investigations on the evolution of resistant mechanism and dissemination of the -resistant genes of MRSA. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
14712180
Volume :
19
Issue :
1
Database :
Complementary Index
Journal :
BMC Microbiology
Publication Type :
Academic Journal
Accession number :
136914237
Full Text :
https://doi.org/10.1186/s12866-019-1470-2