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' Candidatus Oscillochloris fontis': a novel mesophilic phototrophic Chloroflexota bacterium belonging to the ubiquitous Oscillochloris genus.

Authors :
Gaisin, Vasil A
Burganskaya, Ekaterina I
Grouzdev, Denis S
Osipova, Natalya S
Ashikhmin, Aleksandr A
Sinetova, Maria A
Krutkina, Maria S
Bryantseva, Irina A
Sukhacheva, Marina V
Kochetkova, Tatiana V
Koziaeva, Veronika V
Kalashnikov, Aleksandr M
Gorlenko, Vladimir M
Source :
FEMS Microbiology Letters; Apr2019, Vol. 366 Issue 8, pN.PAG-N.PAG, 1p, 1 Color Photograph, 1 Diagram, 2 Charts, 1 Graph
Publication Year :
2019

Abstract

We present the results of a study of mesophilic anoxygenic phototrophic Chloroflexota bacteria from Mechigmen hot spring (the Chukotka Peninsula) and Siberia. According to 16S rRNA phylogenetic analysis, these bacteria belong to Oscillochloris trichoides. However, sequencing the draft genome of the bacterium from the Chukotka and analysis of the average nucleotide identity, as well as in silico DNA-DNA hybridization, reveal that this bacterium belongs to a novel species within the Oscillochloris genus. We, therefore, propose ' Candidatus Oscillochloris fontis' as a novel taxon to represent this mesophilic alkaliphilic anaerobic anoxygenic phototrophic bacterium. Spectrophotometry and high-performance liquid chromatography analysis show that the bacterium possesses bacteriochlorophylls c and a, as well as lycopene, β-carotene and γ-carotene. In addition, transmission electron microscopy shows the presence of chlorosomes, polyhydroxyalkanoate- and polyphosphate-like granules. The genome of ' Ca. Oscillochloris fontis' and the Siberian strains of Oscillochloris sp. possess the key genes for nitrogenase complex (nif H) and ribulose-1,5-bisphosphate carboxylase/oxygenase (cbb L), as previously described for O. trichoides DG-6. The results presented here, and previously published data, show that Oscillochloris bacteria from different aquatic environments have the potential for CO<subscript>2</subscript> and N<subscript>2</subscript> fixation. Additionally, we describe a new primer system for the detection of RuBisCo form I. [ABSTRACT FROM AUTHOR]

Details

Language :
English
ISSN :
03781097
Volume :
366
Issue :
8
Database :
Complementary Index
Journal :
FEMS Microbiology Letters
Publication Type :
Academic Journal
Accession number :
136678600
Full Text :
https://doi.org/10.1093/femsle/fnz097